LAST PUBLICATIONS
Gema Bru1, Aaron Navarro1 y Antonio Martínez-Murcia1, 2 * 1 Genetic PCR Solutions™, 03206-Elche 2Universidad Miguel Hernández, 03300-Orihuela, Alicante, España.
2· Impact of beef extract used for sample concentration on the detection of Escherichia coli DNA in water samples via qPCR
Bernardino Machado-Moreira1,2, Silvia Monteiro3, Ricardo Santos3, Antonio Martinez-Murcia4, Andreja Rajkovic5,6, Nada Smigic5, Karl G. Richards7, Florence Abram2, Catherine M. Burgess - 1 Teagasc Food Research Centre, Ashtown, Dublin, Ireland, 2 Functional Environmental Microbiology, National University of Ireland Galway, Galway, Ireland, 3 Laboratório de Análises, Instituto Superior Técnico, Universidade de Lisboa, Lisbon, Portugal, 4 University Miguel Hernandez and Genetic PCR SolutionsTM, Alicante, Spain, 5 Department of Food Safety and Food Quality Management, University of Belgrade-Faculty of Agriculture, Serbia, 6 Department of Food Technology, Safety and Health, Faculty of Bioscience Engineering, Ghent University; Belgium, 7 Teagasc Johnstown Castle Environmental Research Centre, Wexford, Ireland
3 · Internal validation of GPS™ MONODOSE CanAur dtec-qPCR kit following the UNE/EN ISO/IEC 17025:2005 for detection of the emerging yeast Candida auris
Antonio Martínez-Murcia1,2 Aaron Navarro2 Gema Bru2 Anuradha Chowdhary3 Ferry Hagen4,5 Jacques F. Meis4,6 (1)Department of Microbiology, University Miguel Hernández, Orihuela, Alicante, Spain (2)Genetic PCR Solutions™, Elche, Alicante, Spain (3)Department of Medical Mycology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India (4)Department of Medical Microbiology and Infectious Diseases, ECMM Excellence Center, Canisius-Wilhelmina Hospital (CWZ), Nijmegen, The Netherlands (5)Department of Medical Mycology, Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands (6)Centre of Expertise in Mycology Radboudumc/CWZ, Nijmegen, The Netherlands
David Navarro, DanaGen-BioTed S.L, Barcelona, SPAIN, A.Navarro, A. Martinez-Murcia, Genetic PCR Solutions™, Alicante, SPAIN , Adriana Lasa, Hospital de Sant Pau I de la Santa Creu, Barcelona, SPAIN (October 2015)
10 · Certifcation by Genova University of GPS™ qPCR Kits
11 · Detección discriminativa de Mycoplasma gallisepticum y la cepa vacunal 6/85 mediante qPCR
Antonio Martínez-Murcia1,2, Aarón Navarro2, Gema Bru2, Rosa Amejiras3 – 1Universidad Miguel Hernández. 2genetic PCR solutions™ 03206 Elche, Alicante. 3Trow Nutrition (Nutreco) 28760 Tres Cantos, Madrid.
12 · Aeromonas lusitana sp. nov., Isolated from Untreated Water and Vegetables
Antonio Martínez-Murcia, Roxana Beaz-Hidalgo, Aaron Navarro, Mª. João Carvalho, Max Aravena-Román, Antonio Correia, Mª. José Figueras, Mª. José Saavedra
13 · Molecular Diagnostics by Genetic Methods
This chapter deals with most, if not all, genetic methods used for classification and molecular diagnostics of Aeromonas species. These methods include currently used strategies designed for identifying strains at the species level from pure cultures, for detection of species on complex samples, e.g. waters or food, and for genotyping strains. The genus Aeromonas represents one of the best examples to illustrate applications of molecular biology techniques in microbiology, as it early took the benefits from cutting-edge molecular biology developments to be deeply renovated and, at the same time, represents a particularly difficult example of application for molecular diagnostic microbial methods due to its complex historical taxonomy, recent changes, increasing number of new species descriptions, and their extremely close phylogenetic relationships. We have reviewed past, current and new genetic methods, aiming to give an overview and opinions about advantages and disadvantages, performance and limitations. After a brief description of the principles of the procedures, each method is illustrated by applications either in clinical diagnosis, microbial ecologic issues, epidemiologic surveys, population diversity studies, or in taxonomy rearrangements and new taxa descriptions. Present trends and future perspectives of new revolutionary technology, expected at short and medium term, are also discussed.
Antonio Martínez-Murcia1,2, Aarón Navarro2, Gema Bru2, Rosa Amejiras3 – 1Universidad Miguel Hernández. 2genetic PCR solutions™ 03206 Elche, Alicante. 3Trow Nutrition (Nutreco) 28760 Tres Cantos, Madrid.