The 2019-nCoV/SARS-CoV TwoTarget dtec-RT-qPCR comprises a series of specific targeted reagents designed for2019 novel Coronavirus and Severe acute respiratory syndrome coronavirus detection by using qPCR. An outbreak of pneumonia, epidemiologically linked to the Huanan Seafood Wholesale Market in Wuhan, was notified to WHO on 31-Dec-2019 by the Chinese Health Authorities. Chinese scientists were able to isolate a novel coronavirus, named 2019-nCoV by the WHO and, a first genome provided on 7th January 2020, was classified as belonging to a beta-coronavirus of group 2B with at least 70% similarity in genetic sequence to SARS-CoV. Coronavirus are enveloped viruses with a positive-sense, single-stranded RNA genome belonging to the Coronaviridae family. Additional genomes of several isolates provided by 6 different labs at different places, were analysed to design a set of primers and a probe fully specific for this 2019 novel Coronavirus (2019-nCoV).
The SARS-CoV is a coronavirus responsible of the severe acute respiratory syndrome, a viral respiratory disease of zoonotic origin. Due to his similarities with the novel coronavirus it should be of relevance to analyse the presence of SARS-CoV to discard the presence of the virus on tested samples. The kit 2019-nCoV/SARS-CoV TwoTarget dtec-RT-qPCR Test consist on two independent assays one specific for 2019-nCoV and another specific for SARS-CoV which excludes the 2019-nCoV.