The CPsV-phI+II TwoTarget dtec-RT-qPCR comprises a series of species-specific targeted reagents designed for detection of all serotypes of Citrus psorosis virus phylotype I + II species by using qPCR. Citrus psorosis virus is a single-stranded RNA virus infecting Citrus plants worldwide. External symptoms can be exhibited in leaves, fruit, bark, trunk, roots and branches. Leaf infection displays chlorotic flecks, or a decrease in chlorophyll/healthy coloring, mottling and spots; fruit may show ring-like chlorotic areas. Citrus plants which have been reported to exhibit this virus are grapefuit, mandarin, orange and tangerine, lemon, pomelo and lime. It is economically crucial in the production and distribution of citrus fruit worldwide. Two different phylogenetic groups (I and II) have been shown from the analysis of data described and deposited in the public databases (enclosed figure). Both groups are targeted by the CPsV-phI+II TwoTarget dtec-RT-qPCR Test.