The FraTulSubsp epPCR comprises a series of specific targeted reagents designed for Francisella tularensis subspecies detection by using epPCR. Francisella tularensis is a non-motile, non-spore forming, Gram-negative, highly contagious bacteria that causes tularemia (or “rabbit fever”) that is contagious to humans. Tularemia can lead to different clinical signs as ulceroglandular, glandular or typhoidal. F. tularensis is a highly contagious bacteria that can be spread from animals to humans, through vectors such as mosquitos and fleas, or from being breathed in from the air. The bacteria infects humans through skin, mucous membranes, lungs, and the gastrointestinal tract. There are four known subspecies of F. tularensis. The subspecies tularensis is found predominantly in North America, is the most virulent of the four known subspecies, and is associated with lethal pulmonary infections. Subspecies holarctica (also known as biovar palearctica) is found predominantly in Europe and Asia, but rarely leads to fatal disease. Subspecies novicida (previously classified as F. novicida) and subspecies mediasiatica are characterized as a relatively non-virulent strains. Genomic differentiation of the four subspecies can be achieved by using published discriminating regions. Due to its low infectious dose, ease of spread by aerosol, and high virulence, F. tularensis is classified as a potential agent of bioterrorism.
Kit Content and Prices
GPS™ primers and probes are sold for research use only
All GPS™ Kits are available in F100 and MONODOSE Format
GPS™ reagents are compatible with all qPCR devices