The FraTul qPCR comprises a series of specific targeted reagents designed for Francisella tularensis detection by using qPCR. Francisella tularensis is a non-motile, non-spore forming, Gram-negative, highly contagious bacteria that causes tularemia (or “rabbit fever”) that is contagious to humans. F. tularensis is a highly contagious zoonotic bacteria that can be spread through vectors, such as mosquitos and fleas, or from being breathed in from the air. It can infect humans through skin, mucous membranes, lungs, and the gastrointestinal tract. There are four known subspecies of F. tularensis with different degrees of virulence and geographical distribution. Due to its low infectious dose, ease of spread by aerosol, and high virulence, F. tularensis is classified as a potential agent of bioterrorism.

Product Supporting Literature

Xie, Z., Liu, Y., Di, D., Liu, J., Gong, L., Chen, Z., Li, Y., Yu, W., Lv, L., Zhong, Q., Song, Y., Liao, X., Song, Q., Wang, H., Chen, H., 2022. Protection Evaluation of a Five-Gene-Deleted African Swine Fever Virus Vaccine Candidate Against Homologous Challenge. Front. Microbiol. 13.

Liu, Y., Xie, Z., Li, Y., Song, Y., Di, D., Liu, Jingyi, Gong, L., Chen, Z., Wu, J., Ye, Z., Liu, Jianqi, Yu, W., Lv, L., Zhong, Q., Tian, C., Song, Q., Wang, H., Chen, H., 2023. Evaluation of an I177L gene-based five-gene-deleted African swine fever virus as a live attenuated vaccine in pigs. Emerg. Microbes Infect. 12, 2148560.

Muzykina, L., Barrado-Gil, L., Gonzalez-Bulnes, A., Crespo-Piazuelo, D., Cerón, J.J., Alonso, C., Montoya, M., 2024. Overview of Modern Commercial Kits for Laboratory Diagnosis of African Swine Fever and Swine Influenza A Viruses. Viruses 16, 505.

Bru, G., Martínez-Candela, M., Romero, P., Navarro, A., Martínez-Murcia, A., 2023. Internal Validation of the ASFV MONODOSE dtec-qPCR Kit for African Swine Fever Virus Detection under the UNE-EN ISO/IEC 17025:2005 Criteria. Vet. Sci. 10, 564.