Multi-locus Phylogenetic Analysis from Bacterial Pure Cultures

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The phylogenetic analysis of housekeeping gene sequences is a robust and unequivocal approach for bacterial species identification from pure cultures. GPS™ counts with 30 years’ experience in the field of bacterial phylogenetic studies (see link for publications) and a recognized reputation in genetic analysis typing methods.

Please send us the pure culture plates with isolated colonies and GPS™ will proceed with the following phylogenetic analysis:

  • Extraction of DNA from a single colony
  • Primer design and PCR amplification of selected gene (gyrB, rpoD, recA,..)
  • Purification of derived amplicon
  • Determination of genetic sequence
  • Screening to resolve sequence ambiguities
  • Comparisons with databases and objective interpretation
  • Alignments and tables of sequence similarities
  • Inference of phylogenetic trees including sequences from species type strains
  • Phylogenetic and taxonomic conclusions

Arbol MLPA

GPS™ also provide genetic typing services for authentication of strains. Genetic typing may be useful in: quality control of microbial based products, fraud detection, new product registration or European patent submission, traceability of food and water pathogens, environmental epidemiology, support for scientific publications

Please, ask for more informationinfo@geneticpcr.com