This innovative product are individual ready-to-use tubes containing all the components needed for detection of specific pathogens: dNTPs, polymerase, pathogen-specific primers and probe, and the appropriate buffer.
MONODOSE advantages
Dispensing aliquots for PCR from a master solution, with addition of primer/probe, has some disadvantages: rupture of the enzyme by freezing/thawing, risks of cross-contamination, fluorophore deterioration by UV light and time consuming. Some benefits are listed below:
Just add your diluted sample to each tube and run qPCR.
As reactions are dehydrated, can be transported at room temperature.
Optimized MONODOSE dtec-qPCR Tests shows no difference compared to the non-dehydrated ordinary qPCR.
Our HotStart Taq polymerase is very stable in absence of water and is rapidly activated with only 1 minute at 95 ºC.
Thermal regime is quick and the same for all our kits, you may perform detection of different pathogens in the same run.
Compatibility with all qPCR devices, plate based and glass capillary.
Take the MONODOSE tubes you need to test from the fridge, no need defrosting, no risk of contamination...
...Just add sample and run PCR with our standard protocol.
MONODOSE PRICES
DNA Target
·M24 (24 DOSE + Standard) 148€
·M96 (96 DOSE + Standard) 398€
RNA Target
·M60 (60 DOSE + Standard) 322€
·M120 (120 DOSE + Standard)584€
Technical Review
Our optimized MONODOSE dtec-qPCRkits show no difference compared to the non-dehydrated ordinary qPCR.
Our HotStart Taq polymerase is very stable in absence of water and is rapidly activated in only 1 minute at 95ºC.
MONODOSE dtec-qPCRoptimised to give the best performance and faster.
Fig. 1. MTplex qPCR-test by dispensing ordinary mix (grey) and, curves obtained with Monodose qPCR-Test (red)
Experiments performed with MONODOSE dtec-qPCRdemonstrated that is stable at least up to 7 days at room temperature. (Fig.2. sensitivity and intensity remains)
MONODOSE dtec-qPCR may be transported at room temperature reducing shipping and disposal costs but also making our product friendly to the environment.
Fig. 2. Kinetics obtained from the MTplex qPCR-test by dispensing ordinary mix (Grey) or by using the MONODOSE dtec-qPCR at day of preparation (red)day 0; 2nd day(yellow); day 4 ( green); and after 7 days (blue).
Reproducibility was assessed after 2, 4, and 7 days maintained at room Tª. As showed in Figure 3, assayed points 102, 104, and 106 (genomic copies), showed no dispersion
Thermal regime is quick and the same for all our kits: you may perform detection of different pathogens in the same run.
Fig. 3. Calibration curve of the MTplex qPCR-test by dispensing ordinary mix (Grey) or by using the MONODOSE dtec-qPCR at first day of preparation (red) day 0; 2nd day (yellow); day 4 (green); and after 7 days (blue). Assayed points showed no dispersion
Thermal regime is quick and the same for all our kits: you may perform detection of difoptimized MONODOSE dtec-qPCRkits show no difference compared to the non-dehydrated ordinary qPCR
*GPS primers and probes are sold for research use only.
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104 copies
102 copies
Our HotStart Taq polymerase is very stable in absence of water and is rapidly activated in only 1 minute at 95ºC.
MONODOSE Dtec-qPCRoptimised to give the best performance and faster.
Fig. 1. MTplex qPCR-test by dispensing ordinary mix (grey) and, curves obtained with Monodose qPCR-Test (red)
106 copies
104 copies
Experiments performed with MONODOSE Dtec-qPCRdemonstrated that is stable at least up to 7 days at room temperature. (Fig.2. sensitivity and intensity remains).
102 copies
MONODOSE Dtec-qPCR may be transported at room temperature reducing shipping and disposal costs but also making our product friendly to the environment.
Fig. 2. Kinetics obtained from the MTplex qPCR-test by dispensing ordinary mix (Grey) or by using the MONODOSE Dtec-qPCR at day of preparation (red) day 0; 2nd day (yellow); day 4 ( green); and after 7 days (blue).
Reproducibility was assessed after 2, 4, and 7 days maintained
at room Tª. As showed in Figure 3, assayed points 102, 104, and
106 (genomic copies), showed no dispersion
Fig. 3. Calibration curve of the MTplex qPCR-test by dispensing ordinary mix (Grey) or by using the MONODOSE Dtec-qPCR at firstday of preparation (red) day 0; 2nd day (yellow); day 4 ( green); and after 7 days (blue). Assayed points showed no dispersion.