Bacterial Porcine Diarrhoea
Description
The Bacterial Porcine Diarrhoea dtec-qPCR-Panel-3 comprises a series of specific targeted reagents designed for Brachyspira hyodysenteriae, Lawsonia intracellularis and Brachyspira pilosicoli detection by using qPCR. Enteric bacterial infections are among the most common and economically significant diseases affecting swine production worldwide. Clinical signs of these infections include diarrhoea, reduced growth rate, weight loss, and death of preweaned, weanling, grower-finisher, young and adult age breeding animals. This panel was designed to detect three microorganisms related with the bacterial swine diarrhoea. Brachyspira hyodysenteriae (formerly Treponema hyodysenteriae and Serpulina hyodysenteriae) is a Gram-negative, motile, loosely coiled spirochete belonging to Brachyspiraceae family. This pathogenic bacteria colonizes the large intestine of pigs causing dysentery. Swine dysentery is easily spread by the faecal-oral route due to the ability of B. hyodysenteriae to survive several weeks in moist faeces from infected pigs. Brachyspira pilosicoli (formerly Serpulina pilosicoli) is a Gram-negative, spiral-shaped, obligate anaerobe bacterium member of the Spirochaete family. It causes intestinal spirochaetosis in pigs. Infection of B. pilosicoli is acquired the faecal-oral route. Once in the alimentary tract, the bacterium produce oedema, haemorrhage and the infiltration by inflammatory cells. Lawsonia intracellularis is a Gram-negative, obligated intracellular bacterium belonging to Desulfovibrionaceae family. It causes intestinal hyperplasia mainly in pigs. The disease has two clinical manifestations in pigs: an acute hemorrhagic diarrhoea and sudden death of pigs close to market age often called proliferative hemorrhagic enteropathy; and a chronic mild diarrhoea with poor growth performance in grower-finisher pigs referred as porcine intestinal adenomatosis.
Kit Content and Prices
GPS™ primers and probes are sold for research use only
All GPS™ Kits are available in F100 and MONODOSE Format
GPS™ reagents are compatible with all qPCR devices