The BraPil dtec-qPCR comprises a series of specific targeted reagents designed for Brachyspira pilosicoli detection by using qPCR. Brachyspira pilosicoli (formerly Serpulina pilosicoli) is a Gram-negative, spiral-shaped, obligate anaerobe bacterium member of the Spirochaete family. It causes intestinal spirochaetosis in pigs, but it has also been isolated from dogs, birds and mice and also can cause zoonotic infection in humans. Dogs are thought to be a source of infection for people. Infection of B. pilosicoli is acquired the faecal-oral route. Once in the alimentary tract, the bacterium produce oedema, haemorrhage and the infiltration by inflammatory cells. The consequences are the sloughing of cells into the intestinal lumen, malabsorption and secretory diarrhoea. Affected pigs may be inappetent and grow slowly.
Kit Content and Prices
GPS™ primers and probes are sold for research use only
All GPS™ Kits are available in F100 and MONODOSE Format
GPS™ reagents are compatible with all qPCR devices