The CoVID-19 dtec-RT-qPCR comprises a series of specific targeted reagents designed for Severe acute respiratory syndrome coronavirus 2 detection by using qPCR. An outbreak of pneumonia, epidemiologically linked to the Huanan Seafood Wholesale Market in Wuhan, was notified to WHO on 31th December 2019 by the Chinese Health Authorities. SARS-CoV, MERS-CoV, avian influenza, influenza and similar viruses were ruled out. Chinese scientists were able to isolate a novel coronavirus, firstly named 2019-nCoV by the WHO and, a first genome provided on 7th January 2020, was classified as belonging to a beta-coronavirus of group 2B with at least 70% similarity in genetic sequence to SARS-CoV. Due to this similarity the 2019-nCoV was named by the International Committee on Taxonomy of Viruses as Severe acute respiratory syndrome coronavirus 2 (SARS-CoV2). Coronavirus are enveloped viruses with a positive-sense, single-stranded RNA genome belonging to the Coronaviridae family. Additional genomes of several isolates provided by 6 different labs at different places, were analysed to design a set of primers and a probe fully specific for this 2019 novel coronavirus.
Standard DNA was used to obtain the calibration curve and the retrotranscription step was verified in parallel with synthetic RNA.