The MPXV&clade1b dtec-qPCR Duplex comprises a series of specific targeted reagents designed for Monkeypox virus clade 1b and Monkeypox virus detection by using multiplexed qPCR. Monkeypox virus is a double-stranded DNA virus belonging to the Orthopoxvirus genus in the Poxviridae family. Monkeypox virus causes disease in various animals, including humans. Symptoms begin with fever, headache, muscle pains, swollen lymph nodes, and feeling tired. This is followed by a rash that forms blisters and crusts over. Transmission occurs primarily through contact with infected animals or their bodily fluids. Human-to-human transmission happens via direct skin-to-skin contact, respiratory droplets, or contact with contaminated objects like clothing or bedding. Monkeypox is endemic in tropical rainforest regions of Central and West Africa but, since 2003, some outbreaks occur periodically in different countries around the world. There are two distinct clades of the virus: clade 1 (with subclades 1a and 1b) and clade 2 (with subclades 2a and 2b). In August 2024, outbreaks of clade 1b were detected beyond Africa. Monkeypox virus clade 1b is of particular concern due to its higher transmissibility, more severe clinical outcomes and mortality rate of 4%. The World Health Organization (WHO) declared monkeypox a public health emergency of international concern twice, first on May 2022 and the second time on August 2024. The assay can detect all the clades of the Monkeypox virus and differentiate positives for the clade 1b.