The VibChoTx dtec-qPCR comprises a series of species-specific targeted reagents designed for detection of pathogenic strains of Vibrio cholerae by using qPCR. Vibrio cholerae is a Gram-negative, facultatively anaerobic, comma-shapedbacterium, and has a flagellumat one cell pole. Some strains of V. choleraecause the diseasecholera. V. choleraewas first isolated as the cause ofcholeraby Italian anatomist Filippo Paciniin 1854, but his discovery was not widely known until Robert Koch published the knowledge and the means of fighting the disease. V. cholerae pathogenicity genes code for proteins directly or indirectly involved in the virulence of the bacteria. During infection, V. cholerae secretescholera toxin, aproteinthat causes profuse, watery diarrhea. Colonization of the small intestine also requires the toxin coregulated pilus, a thin, flexible, filamentous appendage on the surface of bacterial cells.

Kit Content and Prices

F100

MONODOSE