The MycAga qPCR comprises a series of specific targeted reagents, for Veterinary diagnostic, designed for Mycoplasma agalactiae detection by using qPCR. Mycoplasma agalactiae is a species of bacteria in the genus Mycoplasma, characterized for the lack of a cell wall around their cell membrane. It can be present in the milk of sheep and goats and can cause mastitis in the animal leading to a loss of milk production, arthritis, and infectious keratoconjunctivitis. The disease is known as contagious agalactia, and despite it is principally caused by M. agalactiae, in recent years, M. mycoides subsp. capri, and, to a lesser extent, M. capricolum subsp. capricolum and M. putrefaciens have also been isolated from goats with mastitis and arthritis. In serious outbreaks with infections with M. agalactiae, whole herds have been lost.