The CSFV RT-epPCR comprises a series of species-specific targeted reagents designed for Classical swine fever virus detection by using epPCR. Classical swine fever virus, previously called Hog cholera virus (species Orthopestivirus suis), is a single-stranded RNA virus of the family Pestiviridae. It is the causative agent of classical swine fever, a highly contagious disease affecting domestic pigs and wild boar. The clinical manifestations of the disease vary according to the virulence of the strain and may include high fever, lethargy, anorexia, haemorrhages, cyanosis, respiratory and gastrointestinal signs, reproductive disorders and high mortality, particularly in acute infections. The virus is transmitted through direct contact with infected animals, ingestion of contaminated feed or pork products, and exposure to contaminated fomites or biological materials. Although many countries and regions have successfully eradicated classical swine fever through surveillance and control programmes, it remains endemic in parts of Asia, Latin America, and other areas. The assay design is based on the method developed by Hoffmann et al. (2005), which is widely used for the detection of the virus.

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F100