Foodborne Poisoning
Description
The Foodborne Poisoning dtec-qPCR-Panel-4 comprises a series of specific targeted reagents designed for Salmonella spp., Listeria monocytogenes and Escherichia coli Shiga toxin 1 and 2 detection by using qPCR. Foodborne poisoning is any illness resulting from food contaminated with a disease-causing agent as pathogenic bacteria, viruses, or parasites. Symptoms often include vomiting, fever, and aches, and may include diarrhea. Foodborne illness usually arises from improper handling, preparation, or food storage. Good hygiene practices before, during, and after food preparation can reduce the chances of contracting an illness. As bacteria are a common cause of foodborne illness, there are three main targets controlled by food safety normatives: Salmonella spp., Listeria monocytogenes and more recently the shigatoxigenic group of Escherichia coli (STEC). Salmonella is closely related to the Escherichia genus and are found worldwide in cold and warm-blooded animals (including humans), and in the environment. Salmonella species are facultative intracellular pathogens that enter cells via macropinosomes. L. monocytogenes is the bacterium that causes the infection listeriosis. It can grow and reproduce inside the host’s cells and is one of the most virulent food-borne pathogens, with 20 to 30 percent of clinical infections resulting in death. Shiga toxin is a family of related toxins with two major groups, Stx1 and Stx2. The most common sources for Shiga toxin are the bacteria Shigella dysenteriae and the shigatoxigenic group of Escherichia coli (STEC), which includes serotypes O157:H7, O104:H4, and other enterohemorrhagic E. coli (EHEC). Shiga toxins have many names, which can be used interchangeably (shiga toxins, shiga-like toxins, cytotoxins, verocytotoxins, verotoxins). STEC live in the guts of ruminant animals, including cattle, goats, sheep, deer, and elk.
Kit Content and Prices
GPS™ primers and probes are sold for research use only
All GPS™ Kits are available in F100 and MONODOSE Format
GPS™ reagents are compatible with all qPCR devices