The MycGal&Mg 6/85 dtec-qPCR Duplex contains individual ready-to-use tubes containing all the components needed for M. gallisepticum vaccine strain 6/85 and Mycoplasma gallisepticum detection by using multiplexed qPCR. Mycoplasma gallisepticum is a bacterium belonging to the family Mycoplasmataceae. It is the causative agent of chronic respiratory disease in chickens and infectious sinusitis in turkeys, chickens, game birds, pigeons, and passerine birds of all ages. These diseases affect chickens and turkeys worldwide, causing the most significant economic losses in large commercial operations. M. gallisepticum is transmitted vertically within some eggs from infected breeders to progeny, and horizontally via infectious aerosols and through contamination of feed, water, and the environment. Most commercial flocks are M. gallisepticum free, however, the disease is most frequently introduced from non-commercial flocks. M. gallisepticum strain 6/85 is the most widely used live vaccine for M. gallisepticum, thus differentiation from pathogenic strains is essential for disease control in farms. The use of this duplex, allows the confirmation of the presence of M. gallisepticum by discarding a positive result due to the vaccine strain 6/85.