The AnaSpp&TrySub dtec-qPCR Duplex contains individual ready-to-use tubes containing all the components needed for Anaplasma spp. and Trypanozoon subgenus (T. evansi, T. brucei, T. equiperdum) detection by using multiplexed qPCR. Anaplasma is a genus of rickettsial bacteria belonging to the Ehrlichiaceae family. Anaplasmas live in the blood cells of the host and cause the disease anaplasmosis. An intermediate tick host is required for maturation. One of the major consequences of infection with Anaplasma is the development of haemolytic anaemia due to the damage done to red blood cells. Some species within the genus of veterinary interest include A. marginale, A. centrale in cattle; A. mesaeterum, A. ovis in sheep and goats and A. phagocytophilum in dogs, cats and horses. A. phagocytophilum can also infect humans.

Trypanosomes are unicellular, eukaryotic parasites of the mammalian bloodstream that cause a major health and economic burden in communities with endemic circulating strains. The subgenus Trypanozoon is composed by the species Trypanosoma brucei, responsible of nagana and sleeping sickness; T. evansi, causes surra; and T. equiperdum, responsible for horse dourine. The species within the Trypanozoon taxa are morphologically indistinguishable, with the main differences between them being the host or the insect vector that enables their distribution.