The OstSex epPCR comprises a series of species-specific targeted reagents designed for determination of sex in ostrich by using epPCR. Sex determination in ostrich is sometimes difficult due to the absence of sexual dimorphism in many species. The use of genetic markers to determine the sex of ostriches is the most effective alternative for accuracy and speed. Sex determination in young ostriches is of interest in ex situ conservation programs, production, operation, and population studies.
GPS™ has designed PCR primers for specific amplification of a conserved region only present in the female DNA of ostrich, contained in tubes named OstSex epPCR-mix. This arrangement specifically gives a single fragment amplification (displayed in a single band by electrophoresis in agarose gels) of 230 bp size in the females, but no amplification products should be obtained from the males. GPS™ also designed primers to detect the ostrich genus (Struthio spp.), contained in the tubes named OstSpp epPCR-mix. This PCR reaction should specifically give an amplified band of 120 bp which ensures that DNA from ostrich has been extracted and added to PCR.
Kit Content and Prices
GPS™ primers and probes are sold for research use only
All GPS™ Kits are available in F100 and MONODOSE Format
GPS™ reagents are compatible with all qPCR devices